Composite

Part:BBa_K4179008:Design

Designed by: Yasmin Habib   Group: iGEM22_Technion-Israel   (2022-10-05)


PsPT1 under Rhlr promoter + P2A + mCherry reporter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 271
    Illegal EcoRI site found at 706
    Illegal XbaI site found at 2149
    Illegal PstI site found at 199
    Illegal PstI site found at 1789
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 271
    Illegal EcoRI site found at 706
    Illegal NheI site found at 1354
    Illegal PstI site found at 199
    Illegal PstI site found at 1789
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 271
    Illegal EcoRI site found at 706
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 271
    Illegal EcoRI site found at 706
    Illegal XbaI site found at 2149
    Illegal PstI site found at 199
    Illegal PstI site found at 1789
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 271
    Illegal EcoRI site found at 706
    Illegal XbaI site found at 2149
    Illegal PstI site found at 199
    Illegal PstI site found at 1789
    Illegal AgeI site found at 1428
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The linker (BBa_K4179004) contains a 6xHis tag and a thrombin recognition and cleavage site.